Handbook of Biomedical Nonlinear Optical MicroscopyBarry R. Masters, Peter So Oxford University Press, USA, 2008 M05 19 - 860 pages The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections.The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing. |
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acceptor molecule amplitude anti-Stokes applications atom beam biological CARS cellular coefficient coherent collagen confocal microscopy cross-section dendritic detection detector diffusion dipole Dirac dispersion dynamics effect efficiency electron emission equation excitation microscopy femtosecond femtosecond laser fiber Figure fluorescence correlation spectroscopy fluorescence lifetime fluorescence lifetime imaging fluorescence microscopy fluorophores focal Förster frequency FRET function harmonic intensity interaction König laser laser pulses laser scanning lens Lett lifetime imaging light living cells Maria Göppert-Mayer measured membrane method mode-locked modulation molecular molecules multiphoton excitation multiphoton microscopy NAD(P)H neurons nonlinear microscopy nonlinear optical optical microscopy oscillator perturbation theory phase photobleaching Phys Physics pixel polarization probes protein quantum mechanical quantum theory radiation Raman scattering resolution resonance energy transfer sample second-harmonic signal skin spatial specimen spectral techniques tissue TPEF transition tumor two-photon excitation two-photon excitation microscopy typically vibrational vivo wave wavelength